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Composite

Part:BBa_K208038:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)

Lac Promoter/RBS/GeneIII/PhaP/Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for phasin secretion. The signal peptide sequence and the phasin sequence were both constructed as individual Silver-fusion compatible BioBrick parts.

Source

This is a composite part constructed by repeated ligation steps. The device has been sequenced and appears to be functional.

References

1. Choi JH, Lee SY (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli Appl Microbiol Biotechnol 64:625-635
2. Maehara A, Ueda S, Nakano H, Yamane T (1999) Analyses of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitrificans. J Bacteriol 191:2914-2921 3. Mergulhão, FJM, Summers DK, Monteiro (2005) Recombinant protein secretion in Escherichia coli Biotechnol Adv 23:177-202 4. Phillips I, Silver P (2006) A new biobrick assembly strategy designed for facile protein engineering. 5. Suriyanmongkol P, Weselake R, Narine S, Moloney M, Shah S (2007) Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants – A review. Biotechnol Adv. 25:148-175 6. York GM, Junker BH, Stubbe J, Sinskey AJ (2001) Accumulation of the PhaP Phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells. J Bacteriol 183:4217-4226